Multiple forms of cytochrome P-450 (III): Inductory mode of cytochrome P-450 from rat liver microsomes treated with phénobarbital
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چکیده
منابع مشابه
Cytochrome P-450 Reductase from Adrenocortical Microsomes
The interaction between P-450czl and NADPH-cytochrome P-450 reductase, both purified from bovine adrenocortical microsomes, has been investigated in a reconstituted system with a nonionic detergent, Emulgen 913, by kinetic analysis and gel filtrations. Steady state kinetic data in progesterone 2 1-hydroxylation showed formation of an equimolar complex between the two enzyme proteins at low Emul...
متن کاملExistence and separation of three forms of cytochrome P-450 from rat liver microsomes.
Rat liver microsomes contain three spectrally distinguishable forms of cytochrome P-450. Each form is identified qualitatively and quantitatively by visible spectral changes that occur when the cytochromes P-450 combine with various ligands, such as cyanide. In addition to cytochrome P-450, rat liver microsomes also contain cytochrome b6, which may be removed from the microsomes by digestion wi...
متن کاملCharacterization of a phenobarbital - inducible cytochrome P - 450 , NADPH - cytochrome P - 450 reductase and reconstituted cytochrome P - 450 mono - oxygenase system from rat brain
Cytochrome P-450 was purified to apparent homogeneity from the brain microsomes of phenobarbital-treated rats. The specific content of the purified P-450 was 12.7 nmol/mg of protein. NADPH-cytochrome P-450 reductase (reductase) was also purified to apparent homogeneity from brain microsomes. The specific content was 34.7,mol of cytochrome c reduced/min per mg of protein. The reduced carbon mono...
متن کاملHighly purified detergent-solubilized NADPH-cytochrome P-450 reductase from phenobarbital-induced rat liver microsomes.
NADPH-cytochrome P-450 reductase was highly purified from liver mlcrosomes of phenobarbital-induced rats by column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, and hydroxylapatite in the presence of deoxycholate or Renex 690, a nonionic detergent. The purified enzyme gave a single major band with a molecular weight of 79,000 daltons on SDS-polyacrylamide gel electrophoresis. FFN and FA...
متن کاملStudies on the synthesis of cytochrome P-450 and cytochrome P-448 in rat liver.
The synthesis of cytochrome P-450 (phenobarbital inducible) and cytochrome P-448 (3-methylcholanthrene inducible) have been studied in rat liver in vivo and in the wheat germ cell-free system using anti-cytochrome P-450 and anti-cytochrome P-448 antibodies. The major mature forms synthesized in vivo correspond to a molecular weight of 47,000 for cytochrome P-450 and 53,000 for cytochrome P-448....
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1983
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)60366-5